Figure 2

Nanostructure-initiator mass spectrometry (NIMS) single-cell FLT-MP/FLT ratiometric imaging. Ratiometric analysis indicates no FLT-MP/FLT intensity in untreated Raji Cells (top right), and significant FLT-MP metabolite production and retention in FLT-treated Raji Cells (bottom). Cells were plated sparsely to ensure that single cells were imaged. FLT, 3′-deoxy-3′-fluorothymidine; FLT-MP, 3′-Deoxy-3′-Fluorothymidine monophosphate.