You are viewing the site in preview mode

Skip to main content

Table 1 Twin-CXnC motif-containing proteins identified in CHCHD4 (shRNA) knockdown cells and CHCHD4 (WT)-expressing cells using SILAC analysis

From: CHCHD4 confers metabolic vulnerabilities to tumour cells through its control of the mitochondrial respiratory chain

Protein Accession (HGNC) CXC motifs Function Mean fold change
CH shRNA/shRNACtrl WT.cl1/control
Coiled-coil-helix-coiled-coil-helix domain-containing 4; CHCHD4 26467 CX9C, CX9C [3] Mitochondrial import [53] 0.62 (****) 4.48 (*)
Coiled-coil-helix-coiled-coil-helix domain-containing 1; CHCHD1 23518 CX9C, CX9C [38] Mitochondrial ribosome [38] 0.86 (***) 1.03
Coiled-coil-helix-coiled-coil-helix domain-containing 2; CHCHD2§ 21645 CX9C, CX9C [54] CIV activity/assembly [8, 54] n.d. 0.63
Coiled-coil-helix-coiled-coil-helix domain-containing 4; CHCHD6§ 28184 CX9C, CX9C [55] Cristae morphology [56] 0.86 1.06
NADH:ubiquinone oxidoreductase subunit A8; NDUFA8§ 7692 CX9C, CX9C [40] CI activity/assembly [8] 0.79 (****) 1.17
NADH:ubiquinone oxidoreductase subunit B7; NDUFB7§ 7702 CX9C, CX9C [40] CI activity/assembly [8] 0.82 (**) 1.12 (*)
NADH:ubiquinone oxidoreductase subunit B10; NDUFB10§ 7696 CX6C, CX11C [57] CI activity/assembly [8, 57] 0.86 (**) 1.13
NADH:ubiquinone oxidoreductase subunit S5; NDUFS5§ 7712 CX9C, CX9C [40] CI activity/assembly [8] 0.81 (***) 1.11
Ubiquinol-cytochrome c reductase hinge protein; UQCRH 12590 CX10C, CX9C [58] CIII assembly/activity [59] 0.96 1.05
C-X9-C motif-containing 1; CMC1 28783 CX9C, CX9C [60] CIV assembly/activity [61] 0.68 (****) 1.10
Cytochrome c oxidase assembly factor 6; COA6§ 18025 CX9C, CX10C [62] CIV assembly/activity [63] 0.70 (****) 2.00 (*)
Cytochrome c oxidase subunit 6B1; COX6B1 2280 CX9C, CX10C [64] CIV assembly/activity [8, 64] 0.81 (**) 1.43 (**)
Cytochrome c oxidase copper chaperone; COX17 2264 CX9C, CX9C [53] CIV assembly/activity [65] n.d. 2.34 (*)
Translocase of inner mitochondrial membrane 8 homologue A; TIMM8A§ 11817 CX3C, CX3C [66] Matrix protein import [8, 67] 0.71 (*) 1.30 (*)
Translocase of inner mitochondrial membrane 8 homologue B; TIMM8B§ 11818 CX3C, CX3C [66] Matrix protein import [67] 0.76 (*) n.d.
Translocase of inner mitochondrial membrane 9; TIMM9 11819 CX3C, CX3C [66] Matrix protein import [67] 0.80 (***) 1.40 (***)
Translocase of inner mitochondrial membrane 10; TIMM10§ 11814 CX3C, CX3C [66] Matrix protein import [8, 67] n.d. 1.07
Translocase of inner mitochondrial membrane 10B; TIMM10B§ 4022 CX3C, CX3C [66] Matrix protein import [67] 0.81 (*) 1.08
Translocase of inner mitochondrial membrane 13; TIMM13§ 11816 CX3C, CX3C [66] Matrix protein import [8, 67] 0.77 (*) 1.47 (*)
  1. Table shows selected proteins, their respective twin-CXnC motif and the fold change in their mitochondrial availability in CHCHD4 (shRNA) knockdown cells compared to control shRNA cells and CHCHD4 (WT)-expressing compared to control cells. Known CHCHD4-binding proteins are indicated (§). Representative mean fold changes in proteins observed in response to CHCHD4 knockdown were calculated from two parallel labelling analyses (CHCHD4 shRNA(H) vs shRNA Ctrl(L) and CHCHD4 shRNA(L) vs shRNA Ctrl(H)) from two independent SILAC experiments. Representative mean fold changes in proteins observed in response to elevated CHCHD4 expression were calculated from two parallel labelling analyses (WT(H) vs Ctrl(L) and WT(L) vs Ctrl(H)) from three independent SILAC experiments for all proteins shown, except CHCHD2 and TIMM8A where data was from two independent SILAC experiments.
  2. Proteins significantly changed are indicated *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Proteins not detected or showing no reproducible change between experiments are indicated (n.d.)
Back to article page

Cancer & Metabolism

ISSN: 2049-3002