Fig. 4

Transcripts expression analysis of the ten most downregulated molecules in MCF7Ecadvar cells relative to MCF7pcDNA3. Quantitative real-time PCR of a CRABP2, YWHAZ, G6PD, HSP90AA1, CTSD, ANXA4; b TPI1, PRDX2, S100A11; and c GGCT in MCF7pcDNA3 and MCF7Ecadvar cells. The relative expression was calculated as described in the “Materials and Methods” section, using GAPDH as the endogenous gene and the MCF7pcDNA3 cell line as reference. *p < 0.05, **p < 0.01, ns not significant