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Table 2 Stoichiometric constants and correction factors used for intact cell JATP estimation

From: Aglycemic growth enhances carbohydrate metabolism and induces sensitivity to menadione in cultured tumor-derived cells

Substrate (S) Max O/mol S Max H+ pumped/mol S ATP from SCS Total ATP (mol S−1) P/O (total ATP/mol O)
d-Glucose - > CO2 (MAS active) 12 110 2 33.45 2.78
Pyruvate - > CO2 5 46 1 13.27 2.654
Glutamine - > CO2 (pyruvate cycle active) 8 62 2 18.9 2.36
  1. ATP/O ratios for substrate refeeding conditions were estimated as previously described [37]. The following values were assumed: 11/3 H+/ATP phosphorylated; 2e-/O; 2NADH (or 2QH2)/O2; 1ATP/GTP at succinyl CoA synthetase (SCS). Malate aspartate shuttle (MAS)
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Cancer & Metabolism

ISSN: 2049-3002

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